This paper measured the impact of the implementation of the five core Industry 4.0 technologies (artificial intelligence, big data analytics, cloud computing, cybersecurity, and Internet of Things (IoT)) on organizational behavior and corporate culture of businesses and organizations in Pakistan. Moreover, the influence of organizational behavior and corporate culture on organizational effectiveness was also analyzed in this paper. The retail industry of Pakistan was chosen as the subject of this study, and a quantitative approach, which included a questionnaire based on a five-point Likert scale ranging from “strongly agree” to “strongly disagree,” was employed to gather the responses from participants, including both superiors and subordinates possessing the technological know-how of Industry 4.0. The findings indicated that the implementation of Industry 4.0 in Pakistan’s retail sector helps positively transform organizational behavior and aids in building a great corporate culture. Moreover, it was found that both positive organizational behavior and great corporate culture help in improving the overall organizational effectiveness. This study took an unprecedented step to research Industry 4.0 from an organizational perspective. Furthermore, the carried-out study extended the theoretical body of knowledge by studying and examining the five crucial factors of Industry 4.0 that contribute to the service sector, particularly the retail industry, of the big emerging markets (BEM) economies, including Pakistan.